Uvavanyo lwegazi luka-ELISA

I-ELISA imele i-immunoassay enxulumene ne-enzyme. Luvavanyo oluqhelekileyo olusetyenziselwa ukufumanisa ii-antibodies ezisegazini. Isilwi-buhlungu yiprotein eveliswa ngamajoni omzimba xa efumanisa izinto eziyingozi, ezibizwa ngokuba ziiantigen.

Isampulu yegazi iyafuneka. Uninzi lwexesha, igazi litsalwa kumthambo ngaphakathi kwengqiniba okanye ngasemva kwesandla.

Isampulu ithunyelwa elebhu apho i-antibody okanye i-antigen ekujoliswe kuyo inxulunyaniswe ne-enzyme ethile. Ukuba into ekujoliswe kuyo ikwisampulu, isisombululo sovavanyo sijika umbala owahlukileyo.

Akukho lungiselelo lukhethekileyo luyafuneka.

Xa kufakwa inaliti ukutsala igazi, abanye abantu baziva iintlungu eziphakathi. Abanye baziva behlaba okanye behlaba kuphela. Emva koko, kunokubakho ukubetha okanye ukukrala kancinci. Oku kuya kuhamba kungekudala.

Olu vavanyo luhlala lusetyenziselwa ukubona ukuba ubonakalisiwe kwiintsholongwane okanye ezinye izinto ezibangela usulelo. Isetyenziselwa ukukhusela usulelo lwangoku okanye oludlulileyo.

Amaxabiso aqhelekileyo axhomekeke kuhlobo lwento echongiweyo. Ezinye iilebhu zisebenzisa imilinganiselo eyahlukeneyo okanye ukuvavanya iisampulu ezahlukeneyo. Amaxabiso esiqhelo ahluka kancinci phakathi kweelebhu ezahlukeneyo. Thetha nomboneleli wakho wezempilo malunga nentsingiselo yeziphumo zakho zovavanyo.

Amaxabiso angaqhelekanga axhomekeke kuhlobo lwento echongiweyo. Kwabanye abantu, isiphumo esihle sinokuqheleka.

Akukho mngcipheko ubandakanyekileyo ekuthatheni igazi lakho. Imithambo kunye nemithambo iyahluka ngobukhulu ukusuka komnye umntu ukuya komnye, kwaye ukusuka kwelinye icala lomzimba ukuya kwelinye. Ukuthatha igazi kwabanye abantu kunokuba nzima ngakumbi kunabanye.

Eminye imingcipheko enxulumene nokutsalwa kwegazi incinci, kodwa inokubandakanya:

• Ukopha kakhulu
• Ukufa isiqaqa okanye ukuziva ungenantloko
• Ukugqobhoza okuninzi ukufumana imithambo
• I-Hematoma (igazi eliqokelela phantsi kwesikhumba)
• Usulelo (umngcipheko omncinci nangaliphi na ixesha ulusu lwaphukile)

I-immunoassay enxulumene ne-enzyme; I-EIA

• Uvavanyo lwegazi

UAoyagi K, Ashihara Y, Kasahara Y.I-Immunoassay kunye ne-immunochemistry. Ku: McPherson RA, Pincus MR, ii-eds. Ukuchongwa kweKlinikhi kaHenry kunye noLawulo ngeendlela zeLebhu. Umhla we-23. ISt Louis, MO: Elsevier; Ngo-2017: isahluko 44.

UMurray PR. Ugqirha kunye nelebhu ye-microbiology. Ku: Bennett JE, Dolin R, Blaser MJ, ii-eds. UMandell, uDouglas, kunye neMigaqo yeBennett kunye nokuSebenza kwezifo ezosulelayo, uHlelo oluHlaziyiweyo. Ngomhla we-8. IPhiladelphia, PA: Elsevier Saunders; 2015: isahluko 16.