Electrophoresis: yintoni, yenzelwe ntoni kwaye yenziwa njani

Umxholo

• Yenzelwe ntoni
• Kwenziwa njani
• Iindidi zeelectrophoresis
• 1. Ihemoglobin electrophoresis
• 2. Iiprotein electrophoresis

I-Electrophoresis yindlela yeelebhu eyenziweyo ngenjongo yokwahlula iimolekyuli ngokobungakanani bazo kunye nentlawulo yombane ukuze kufunyaniswe isifo, ukubonakaliswa kweprotein kungangqinwa okanye kubonwe ii-microorganisms.

I-Electrophoresis yinkqubo elula nexabiso eliphantsi, esetyenziswa kwiinkqubo zelebhu nakwiprojekthi zophando. Ngokwenjongo ye-electrophoresis, kunokuba yimfuneko ukwenza olunye uvavanyo kunye neemviwo ukufikelela kwisifo, umzekelo.

Yenzelwe ntoni

I-Electrophoresis inokwenziwa ngeenjongo ezininzi, zombini kwiiprojekthi zophando kunye nokuxilongwa, kuba yindlela elula nexabiso eliphantsi.Ke, i-electrophoresis inokwenziwa kwi:

• Chonga iintsholongwane, ukungunda, iintsholongwane kunye nezimfimfithi-gazi, kunye nokusetyenziswa okuxhaphakileyo kwiiprojekthi zophando;
• Uvavanyo lobuzali;
• Jonga ukubonakaliswa kweeproteni;
• Chonga utshintsho, ube luncedo ekuchongeni ii-leukemias, umzekelo;
• Hlalutya iintlobo zokujikeleza kwehemoglobin, ube luncedo ekufumaneni isifo searomy cell anemia;
• Vavanya inani leeproteni ezikhoyo egazini.

Ngokwenjongo ye-electrophoresis, kunokuba yimfuneko ukwenza ezinye iimvavanyo ezongezelelweyo kugqirha ukugqibezela isifo.

Kwenziwa njani

Ukwenza i-electrophoresis kufuneka ijeli, enokuthi ibe ye-polyacrylamide okanye i-agarose ngokuxhomekeke kwinjongo, isikhuseli se-electrophoresis kunye ne-vat, isiphawuli sobunzima beemolekyuli kunye nedayi ye-fluorescent, ukongeza kwi-UV okanye izixhobo zokukhanya ze-LED, ekwabizwa ngokuba yi-transilluminator .

Emva kokulungisa ijeli, kufuneka kubekwe into ethile yokwenza imithombo yejeli, eyaziwa ngokuba yikama, kwaye ijeli mayimiswe. Xa ijeli sele ilungile, sebenzisa nje ezo zinto equleni. Ukuza kuthi ga ngoku, isiphawuli sobunzima beemolekyuli kufuneka sibekwe kwelinye lamaqula, ulawulo olululo, into leyo eyaziwayo ukuba yintoni, ulawulo olubi, oluqinisekisa ubunyani bempendulo, kunye neesampulu eziza kuhlaziywa. Zonke iisampulu kufuneka zixutywe nedayi ye-fluorescent, kuba ngale ndlela kunokwenzeka ukuba nombono weebhendi ezikwi-transilluminator.

Ijeli eneesampulu kufuneka ibekwe kumatshini we-electrophoresis, oqulathe isisombululo sesixhasi esithile, emva koko isixhobo sivulwe ukuze kubekho umbane kwaye, ngenxa yoko, umahluko onokubakho, obalulekileyo ngokwahlulwa kwamasuntswana ngokwe kumthwalo wabo kunye nobukhulu. Ixesha lokubaleka kwe-electrophoretic liyahluka ngokwenjongo yenkqubo, kwaye linokuhlala ukuya kwiyure enye.

Emva kwexesha elimiselweyo, kunokwenzeka ukuba ujonge isiphumo se-electrophoretic run ngokusebenzisa i-transilluminator. Xa ijeli ibekwe phantsi kwe-UV okanye ukukhanya kwe-LED, kunokwenzeka ukuba ubone ipateni yokubopha: enkulu le molekyuli, kokukhona iyahamba, ukusondela emthonjeni, ngelixa ukukhanya kweemolekyuli, kokukhona kunokwenzeka.

Ukuze impendulo iqinisekiswe, kunyanzelekile ukuba amabhanti olawulo oluqinisekileyo abonwe kwaye kulawulo olubi akukho nto iboniswayo, kuba kungenjalo luphawu lokuba bekukho ukungcola, kwaye yonke inkqubo kufuneka iphindwe.

Iindidi zeelectrophoresis

I-Electrophoresis inokwenziwa ngeenjongo ezahlukeneyo kwaye, ngokwenjongo yayo, iindidi ezininzi zejel zingasetyenziswa, eyona ixhaphakileyo yile polyacrylamide kunye neagarose.

I-Electrophoresis yokuchonga ii-microorganisms ixhaphakile ukwenziwa kwiilebhu zophando, nangona kunjalo, ngeenjongo zokuqonda isifo, i-electrophoresis inokusetyenziselwa ukufumanisa izifo ze-hematological kunye nezifo ezithi zivele ngokwanda kwenani leeproteni, zibe zezona ntlobo ziphambili ze-electrophoresis:

1. Ihemoglobin electrophoresis

I-hemoglobin electrophoresis yinkqubo yaselebhu eyenziweyo yokuchonga iintlobo ezahlukeneyo zehemoglobin ejikeleza egazini, isenza ukuba sikwazi ukubona ubukho bezifo ezinxulumene ne-hemoglobin synthesis. Uhlobo lwehemoglobin luchongwa kusetyenziswa i-electrophoresis kwi-pH ethile, phakathi kwe-8.0 kunye ne-9.0, kunye nepateni yamabhanti eqinisekisiweyo enokuthelekiswa nephethini eqhelekileyo, evumela ukubonwa kobukho behemoglobins ezingaqhelekanga.

Yenzelwe yona: I-hemoglobin electrophoresis yenziwa ukuphanda kunye nokufumanisa izifo ezinxulumene ne-hemoglobin synthesis, enjenge-sickle cell anemia kunye nesifo se-hemoglobin C, ukongeza ekubeni luncedo ekwahluleni i-thalassemia. Funda indlela yokutolika ihemoglobin electrophoresis.

2. Iiprotein electrophoresis

Iprotein electrophoresis luvavanyo oluceliwe ngugqirha ukuvavanya ubungakanani beeproteni ezijikeleza egazini, kwaye ke, zichonge izifo. Olu vavanyo lwenziwa kwisampulu yegazi, ene-centrifuged ukufumana iplasma, eyinxalenye yegazi, ebandakanya, phakathi kwezinye izinto, yeeproteni.

Emva kwe-electrophoresis, iphethini yeebhendi inokubonwa kwaye, emva koko, igrafu apho ubungakanani beqhezu ngalinye leeprotein lubonakalisiwe, isisiseko sokuchongwa.

Yenzelwe yona: Iprotein electrophoresis ivumela ugqirha ukuba aphande ngeziganeko ezininzi ze-myeloma, ukuphelelwa ngamanzi emzimbeni, i-cirrhosis, ukudumba, isifo sesibindi, i-pancreatitis, i-lupus kunye noxinzelelo lwegazi ngokomzekelo webhendi kunye negrafu eboniswe kwingxelo yoviwo.

Qonda indlela eyenziwa ngayo kunye nendlela yokuqonda isiphumo seprotein electrophoresis.