Yintoni inkcubeko yomchamo nge-antibiotogram, yenziwa njani kwaye yenzelwe ntoni

Umxholo

• Yintoni injongo yenkcubeko yomchamo ngeyeza lonyango
• Usiqonda njani iziphumo
• Uroculture nge-antibiogram Escherichia coli
• Kwenziwa njani

Uroculture ene-antibiotogram luvavanyo lwaselebhu olucelwe ngugqirha olujolise ekuchongeni into ebangela usuleleko lomchamo kwaye yintoni iprofayili yayo yobuntununtunu nokumelana namayeza okubulala iintsholongwane aqhele ukusetyenziselwa ukunyanga usulelo. Ngenxa yoko, kwiziphumo zovavanyo, ugqirha angabonisa eyona ilungileyo yokubulala umntu.

Ukusebenza kolu vavanyo kuhlala kubonisiwe xa umntu ebonisa iimpawu zosulelo lomchamo, nangona kunjalo inokucelwa ukuba emva kokuvavanywa kohlobo lomchamo, i-EAS, ibacteria kunye neleukocyte ezininzi kumchamo zichongiwe, oku kungenxa yokuba olu tshintsho zibonisa usulelo lomchamo, kubalulekile ukuba uchonge ii-microorganism.

Yintoni injongo yenkcubeko yomchamo ngeyeza lonyango

Uvavanyo lwenkcubeko yomchamo kunye ne-antibiotogram isebenza ekuchongeni i-microorganism ejongene notshintsho lomchamo kwaye yeyiphi i-antimicrobial enokusetyenziswa ngokufanelekileyo kumlo wayo.

Olu vavanyo lubonakaliswe ikakhulu kwimeko yosulelo lomchamo, kwaye unoku-odolwa emva kwesiphumo sohlobo 1 lovavanyo lomchamo, i-EAS, okanye xa umntu eneempawu neempawu zosulelo lomchamo, ezinjengentlungu kunye nokutsha xa uchama kunye nomnqweno oqhelekileyo ukuba noPee. Yazi indlela yokuchonga iimpawu zosulelo lomchamo.

Olu vavanyo lusebenza ekuchongeni ubukho kunye neprofayili yobuntununtunu kwii-antimicrobials zezinye iintsholongwane, ezona ziphambili zezi:

• Escherichia coli;
• UKlebsiella pneumoniae;
• UCandida sp.;
• Iproteus mirabilis;
• Pseudomonas spp.;
• I-Staphylococcus saprophyticus;
• IStreptococcus agalactiae;
• I-Enterococcus faecalis;
• Serratia marcenses;
• UMorganella morganii;
• Acinetobacter baumannii.

Ukuchongwa kwezinye iintsholongwane ezinokuthi zihambelane nosulelo lomchamo, njenge I-Chlamydia trachomatis, I-Neisseria gonorrhoeae, Mycoplasma spp. kwaye Gardnerella lobufazi, umzekelo, ixesha elininzi ayenziwa ngenkcubeko yomchamo, kwimeko apho kuye kucelwe ukuba kuqokelelwe imfihlo yelungu lobufazi okanye leepenile ukuze kubonwe i-microorganism kunye ne-antibiotiki, okanye uhlalutyo lomchamo ngeendlela zeemolekyuli.

Usiqonda njani iziphumo

Iziphumo zenkcubeko yomchamo kunye neyeza zentsholongwane zinikwa ngohlobo lwengxelo, apho kuboniswa ukuba ngaba uvavanyo luthe alunayo okanye alunayo kwaye, kwezi meko, apho kwafunyanwa khona i-microorganism, ubuninzi bayo kumchamo kunye neyeza lokubulala iintsholongwane. yayinovelwano kwaye ichasene.

Iziphumo zithathwa njengezingalunganga xa kukho ukukhula kuphela kwesixa esiqhelekileyo seentsholongwane eziyinxalenye yendalo yenkqubo yokuchama. Kwelinye icala, umphumo uba mhle xa kukho ukonyuka kobungakanani bayo nayiphi na into eyinxalenye yentsholongwane yesiqhelo okanye xa ubukho bentsholongwane engaqhelekanga buqinisekisiwe.

Ngokubhekisele kwi-antibiotic, ukongeza ekwaziseni ukuba ngaba i-microorganism inovakalelo okanye ayinyangeki kwi-antibiotiki, ikwabonisa ubuncinci be-Inhibitory Concentration, ekwabizwa ngokuba yi-CMI okanye i-MIC, engqinelana nobuncinci be-anti-antibiotic ekwaziyo ukuthintela ukukhula kwe-microbial, olu lwazi lubaluleke kakhulu kugqirha ukubonisa olona nyango lufanelekileyo.

[uphononongo lokujonga kwakhona]

Uroculture nge-antibiogram Escherichia coli

THE Escherichia coli, kwaziwa njenge E. coli, ibacteria ihlala inxulunyaniswa nosulelo lomchamo. Xa inkcubeko yomchamo ilungile kwintsholongwane, isixa esichaziweyo kumchamo, esihlala ngaphezulu kwe-100,000 iikholoni, siyaboniswa kwingxelo, kwaye zeziphi i-antibiotics ezibuthathaka, njengesiqhelo iPhosphomycin, iNitrofurantoin, Amoxicillin eneClavulonate, iNorfloxacino okanye iCiprofloxacino.

Ukongeza, i-MIC iyaboniswa, ethi kwimeko ye Escherichia coliUmzekelo, kugqityiwe ukuba i-MIC ye-Ampicillin engaphantsi okanye elingana no-8 µg / mL ibonisa ukuba sesichengeni sokuthintela i-antibiotiki, kwaye nokusetyenziswa kwayo kunyango kuyacetyiswa, ngelixa amaxabiso alingana okanye angaphezulu kwama-32 µg / mL bonisa ukuba iintsholongwane ziyanyangeka.

Ke, ngokweziphumo ezifunyenwe yinkcubeko yomchamo kunye neyeza lokubulala iintsholongwane, ugqirha angabonisa olona nyango lululo losulelo.

Kwenziwa njani

Uvavanyo lwenkcubeko yomchamo luvavanyo olulula olwenziwa kwisampulu yomchamo, ekufuneka iqokelelwe kwaye igcinwe kwisitya esifanelekileyo esinikezwe elebhu. Ukwenza ingqokelela, kufuneka kuqala ucoce indawo esondeleyo ngesepha namanzi kwaye uqokelele umchamo wokuqala wosuku, kwaye umntu lowo akawutyesheli umjelo wokuqala womchamo kwaye aqokelele umlambo ophakathi.

Kubalulekile ukuba isampulu isiwe elabhoratri kwisithuba seeyure ezi-2 ukuze ibe nakho ukusebenza kwinkcubeko yomchamo kunye neyeza-zintsholongwane. Kwilabhoratri, isampulu ibekwe kwindawo yenkcubeko ethanda ukukhula kweentsholongwane eziqhelekileyo kumchamo. Emva kwe-24h ukuya kuma-48h, kunokwenzeka ukuqinisekisa ukukhula kwamagciwane kwaye, ngenxa yoko, kunokwenzeka ukuba kwenziwe uvavanyo lokuchongwa kwentsholongwane.

Ukongeza, ukusukela kumzuzu wokuba ukukhula kwe-microorganisms kulwimi lwenkcubeko kuqatshelwe, kunokwenzeka ukujonga inani le-microorganisms, kwaye kungabonakaliswa ukuba kukukoloniyali okanye usulelo, ukongeza ekubeni kunokwenzeka ukwenza i-antibiotic , apho kuvavanywa khona ii-microorganism kwii-antibiotics ezahlukeneyo, kujongwa ukuba zeziphi i-antibiotics ezibuthathaka okanye ezinganyangekiyo. Qonda ngakumbi malunga neyeza lonyango.