Uvavanyo lwegazi lweNitroblue tetrazolium

Uvavanyo lwe-nitroblue tetrazolium lujonga ukuba iiseli ezithile zamajoni omzimba zinokutshintsha ikhemikhali engenambala ebizwa ngokuba yi-nitroblue tetrazolium (NBT) kumbala oluhlaza okwesibhakabhaka.

Isampulu yegazi iyafuneka.

Imichiza i-NBT yongezwa kwiiseli ezimhlophe zegazi elebhu. Iiseli ke ziyavavanywa phantsi kwemicroscope ukubona ukuba imichiza ibenze baba luhlaza okwesibhakabhaka.

Akukho lungiselelo lukhethekileyo luyafuneka.

Xa kufakwa inaliti ukutsala igazi, abanye abantu baziva iintlungu eziphakathi. Abanye baziva behlaba okanye behlaba. Emva koko, kunokubakho ukubetha okanye ukukrala kancinci. Oku kuya kuhamba kungekudala.

Olu vavanyo lwenziwe kwiscreen sesifo esinganyangekiyo segranulomatous. Esi sifo sidluliselwa kwiintsapho. Kubantu abanesi sifo, iiseli ezithile zomzimba azincedi ukukhusela umzimba kwizifo.

Umboneleli wezempilo unokuyalela olu vavanyo kubantu abanosulelo rhoqo emathanjeni, kulusu, kumalungu, emiphungeni nakwamanye amalungu omzimba.

Ngokwesiqhelo, iiseli ezimhlophe zegazi zijika zibe luhlaza okwesibhakabhaka xa kongezwa i-NBT. Oku kuthetha ukuba iiseli kufuneka zikwazi ukubulala iintsholongwane kwaye zikhusele umntu kusulelo.

Amaxabiso esiqhelo ahluka kancinane kwelebhu enye iye kwenye. Thetha nogqirha wakho malunga nentsingiselo yeziphumo zakho zovavanyo.

Ukuba isampulu ayitshintshi umbala xa i-NBT idityaniswa, iiseli ezimhlophe zegazi azikho nto ifunekayo ukubulala iintsholongwane. Oku kunokubangelwa sisifo esinganyangekiyo segranulomatous.

Akukho mngcipheko ubandakanyekileyo ekuthatheni igazi lakho. Imithambo kunye nemithambo iyahluka ngobukhulu ukusuka komnye umntu ukuya komnye nakwelinye icala lomzimba liye kwelinye. Ukuthatha igazi kwabanye abantu kunokuba nzima ngakumbi kunabanye.

Eminye imingcipheko enxulumene nokutsalwa kwegazi incinci, kodwa inokubandakanya:

• Ukopha kakhulu
• Ukufa isiqaqa okanye ukuziva ungenantloko
• Ukugqobhoza okuninzi ukufumana imithambo
• I-Hematoma (igazi eliqokelela phantsi kwesikhumba)
• Usulelo (umngcipheko omncinci nangaliphi na ixesha ulusu lwaphukile)

Uvavanyo lwe-NBT

• Uvavanyo lweNitroblue tetrazolium

Ukuphazamiseka komsebenzi we-phagocyte. Ku: IGoldman L, iSchafer AI, ii-eds. Amayeza eGoldman-Cecil. Umhla wama-25. IPhiladelphia, PA: Elsevier Saunders; 2016: isahluko 169.

IRiley RS. Uvavanyo lwelabhoratri yenkqubo yomzimba yokuzikhusela. Ku: McPherson RA, Pincus MR, ii-eds. Ukuchongwa kweKlinikhi kaHenry kunye noLawulo ngeendlela zeLebhu. Umhla we-23. ISt Louis, MO: Elsevier; Ngo-2017: isahluko 45.