Ibala legram: yenziwe njani kwaye yenzelwe ntoni

Umxholo

• Kwenziwa njani ibala leGram
• Yenzelwe ntoni

Ibala legram, okanye nje iGram, yindlela ekhawulezayo nelula ejolise ekwahluleni iintsholongwane ngokweempawu zodonga lwazo lweseli emva kokuvezwa kweedayi ezahlukeneyo kunye nezisombululo.

Yiyo loo nto, ngokusebenzisa ubala lweGram, kunokwenzeka ukuba uqinisekise, ukongeza kubume bentsholongwane, umbala abawufumanayo, kwaye esi siphumo sibalulekile ekuchazeni ezinye iindlela zokuchonga iintlobo zebacteria kunye nogqirha ukubonisa unyango lokuthintela ngokweempawu ezibonakalayo ezincinci.

Ukubola kwegrama kuhlala kwenziwa rhoqo kwilabhoratri kwaye kuyinxalenye yovavanyo lwebacterioscopy. Qonda ukuba yintoni i-bacterioscopy kwaye yenziwa njani.

Kwenziwa njani ibala leGram

Ibala legram yindlela ekhawulezayo, esebenzayo nexabiso eliphantsi yokuchonga iintsholongwane ezinoxanduva losulelo, iluncedo koogqirha ukubonisa unyango lokuthintela usulelo olunokuthi lwenzeke, kuba iimpawu ezithile zala maqela ebhaktiriya ziyaziwa,

Ukubekwa kwegram kwenziwa ngamanyathelo aphambili ayi-7, nangona kunjalo umthetho olandelwayo ungahluka ngokuxhomekeke kwilabhoratri:

1. Beka ezinye iikholoni zebacteria kwisilayidi, ukongeza ithontsi lamanzi ukulungiselela ulungelelwaniso lweekholoni;
2. Yiyeke yome kancinci, kwaye incakuba inokudlula ngokukhawuleza kwilangatye ukuze ithande ukomiswa, nangona kunjalo kubalulekile ukuthathela ingqalelo iqondo lobushushu, kuba ukuba iqondo lobushushu liphezulu kakhulu kunokwenzeka ukuba kukho utshintsho kulwakhiwo iibhaktheriya, ezinokuphazamisa iziphumo zoviwo;
3. Xa isilayidi somile, gubungela ngombala we-violet yikristale kwaye mayiyeke isebenze malunga nomzuzu omnye;
4. Hlamba isilayidi ngomjelo wamanzi abalekayo kwaye uvale isilayidi nge-lugol, enenjongo yokulungisa idayi eluhlaza, kwaye mayibe ngumzuzu omnye. Zombini ezi ntlobo zebacteria ziyakwazi ukufunxa izinto ezintsonkothileyo ezenziwe yidayi kunye ne-lugol, zijika zibe luhlaza okwesibhakabhaka;
5. Emva koko, hlamba isilayidi ngamanzi abalekayo kwaye ufake i-95% yotywala, uyiyeke isebenze imizuzwana engama-30. Utywala bunoxanduva lokunyibilikisa inwebu yelipid eyenza i-gram-negative bacteria kwaye, ngenxa yoko, isuse ubunzima obenziwe phakathi kwedayi kunye ne-lugol, ibala la mabacteria. Nangona kunjalo, kwimeko ye-gram-positive bacteria, utywala bunyibilikisa udonga lweseli lwe-bacteria ene-gram-positive, obangela ukuba ii-pores zingene kwaye zibenze bangabinamandla;
6. Emva koko, kufuneka ihlanjwe kwakhona phantsi kwamanzi abalekayo kwaye igubungele isilayidi ngedayi yesibini, i-fuchsin okanye i-safranin kwaye mayiyeke isebenze imizuzwana engama-30;
7. Emva koko, hlamba isilayidi phantsi kwamanzi abalekayo kwaye uyivumele yome kubushushu begumbi.

Nje ukuba isilayidi somile, kunokwenzeka ukubeka ithontsi lokuntywilisela ioyile kwaye ujonge isilayidi phantsi kwemicroscope ngenjongo eyi-100x, ukubanakho ukujonga ubukho okanye ukungabikho kwebhaktheriya, kunye nobukho begwele kunye neeseli ze-epithelial.

Yenzelwe ntoni

Ibala legram linenjongo ephambili yokwahlula ibacteria ngokweempawu zodonga lweseli kunye ne-morphology ngokubanzi. Ke, ngokweempawu eziqwalaselwe phantsi kwemicroscope, iibhaktheriya zinokuhlelwa zibe:

• Iibhaktheriya ze-Gram, eziboniswa ngombala oluhlaza okwesibhakabhaka ngenxa yokuba azitshintshwanga ngotywala, kuba zinodonga lwesisele esityebileyo kwaye ii-pores zazo ziyaxinana xa ziveliswe kwi-lugol;
• Iibhaktheriya ze-Gram-negative, eziboniswa ngombala opinki / omfusa ngenxa yokuba zinombala otywala zaza zadyojwa yi-safranin okanye i-fuchsin.

Emva kokubona iintsholongwane phantsi kwemicroscope, kunokwenzeka ukuba ezinye iimvavanyo ziya kwenziwa elebhu ukufumanisa iintlobo zebacteria. Nangona kunjalo, ngeGram kunye nokunxulumana neempawu kunye neempawu ezivezwe ngumntu, ugqirha angabonisa unyango lothintelo de kube kufumaneke iziphumo zeemviwo ezithile, kuba ngale ndlela kunokwenzeka ukuba kuncitshiswe izinga lokuphindaphindeka kwebhakteria kwaye kuthintele iingxaki.